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The cells were cultivated in IMDM with glutamine and HEPES (Gibco) with 10% heat inactivated FBS (Gibco) in T75 or T175 flasks (Sarstedt) at 37 C in humidified atmosphere containing 5% CO 2

Jiang, Y., Zhang, G., Wang, J

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Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice

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The Schedule: Usually, youll start with three sessions per week during the loading phase, eventually moving to once-a-week maintenance