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The cells were stained for ROS (S0033M

Lang KS, Lang PA, Bauer C, Duranton C, Wieder T, Huber SM, et al

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and one of them was spiked with a known amount of pro-BDNF or mBDNF, depending on the isoform indicated by the ELISA manufacturer

Saline adds a different problem: the sodium chloride can interact with some peptide chemistries and provides no bacteriostatic activity

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