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Then, islets were handpicked and cultured at 37 C and 5% CO 2 in RPMI 1640 media (Thermo Fisher Scientific, #11875093) containing 11 mM glucose and supplemented with 5 g/l BSA fraction V (Roche, #107351080001), 100 units/ml penicillin and 100 g/ml streptomycin (1% P/S) (Thermo Fisher Scientific)

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5 e Srie Ota MTY (1939) Naevus fusco-coeruleus ophthalmo-maxillaris

Either a pan HLA class II antibody (Tu39) capable of binding a wide subset of HLA-DR1/3/5, -DQ, and -DP molecules or HLA-DR antibody (L243) was used for immunoprecipitation prior to MS analysis