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StablerSPSteegbornCWahlMCOliveriusovaJKrausJPAllenRHet alElevated plasma total homocysteine in severe methionine adenosyltransferase I/III deficiency

An OA mouse model was successfully established by injecting monosodium iodoacetate (MIA) in mouse knee joints 25 , specifically, involving anesthetic administration with 1.25% tribromoethanol (Bimake, M2920) at a dose of 0.2 mL/10 g body weight, followed by intra-articular injection of 10 L MIA (50 mg/ml, Sigma-Aldrich, 57858-25G-F) into the right knee joint using a 30-gauge needle

Helps fight infections and sickness

It also helps transport amino acids across cell membranes, supporting overall metabolic function

Not even for drawing water

That concentration required careful measurement using an insulin syringe, and even small errors could result in significantly wrong dosing