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Reconstituted solutions should be protected from reducing agents (such as DTT or beta-mercaptoethanol), which can cleave the disulfide bond and disrupt the active conformation

Epub 2009 Jan 16

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Effects of chronic ethanol feeding on rat hepatocytic glutathione

Further, overproduction of free radicals have also been shown to occur due to spontaneous breakdown (autoxidation) and/or excessive metabolism by monoamine oxidase of adrenaline, noradrenaline and dopamine, which can further overwhelm the brains antioxidant systems (Cohen, 1984

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