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Annu Rev Immunol (2020) 38:289313

In addition, intestinal glutathione-S-transferase (GST) can catalyze the same reaction ( Gut microbiota-produced erucin is metabolized similarly to SFN, as shown in Figure 2

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The advantage of this approach lies in its fast release speed and strong selectivity, but it is essential to ensure low enzyme levels in normal tissues to avoid off-target release

In fibroblast models, researchers observed that GHK-Cu is associated with increased collagen type I and III synthesis, fibronectin production, and glycosaminoglycan deposition

Western blotting The hippocampus tissue extracts were prepared using Protein extraction reagent (Thermo Fisher Scientific, 78501) and centrifuged at 12,000 rpm for 10 min at 4 C