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Induction of glutathione S-transferase genes of Nicotiana benthamiana following infection by Colletotrichum destructivum and C

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To test this, we took advantage of the reversible nature of disulfide bond formation and incubated the 8 modified cysteine-SpyCatcher003 mutants with 10 mM TCEP (tris(2-carboxyethyl)phosphine), a common reducing agent