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Integrating network pharmacology and experimental validation to clarify the anti-hyperuricemia mechanism of cortex phellodendri in mice

The resulting fluorescence was collected using a 63/1.4 NA oil immersion objective and detected on a 34-channel spectral GaAsP array detector with the gain set at 650 V and split into two ranges of 490632 nm and 641694 nm

This forms a complete regulatory cascade:NNMT inhibition NAD+ elevation metabolic optimization This unique mechanism makes 5-amino-1-methylquinolinium injection a critical tool linking NNMT activity, nicotinamide metabolism, and systemic metabolic homeostasis, providing direct experimental support for exploring NNMT's role in energy metabolism

At acidic pH (below 5), deamidation occurs through direct hydrolysis and is generally slower