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Subsequently, an equal volume of 50 L of the tissue extract and 50 L of sterile saline solution was amalgamated with a corresponding quantity of the Griess reagent, The reactants were by incubation for 30 min at temperature of 37C

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Suspension nasal sprays require all of the same device performance tests as solution sprays, plus drug particle size distribution (DPSD) by cascade impactor because it is the drug particle size (not the droplet size) that determines absorption rate in the nasal mucosa for suspensions