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Statistical Analysis Each assay was repeated independently at least three times to ensure the reliability of this study and the measurement data are expressed as the mean SD

Real-time quantitative PCR, triglycerides, and Oil Red O staining Intramuscular preadipocytes isolation and plasmid construction Intramuscular preadipocytes of the longissimus dorsi muscle were isolated from calves of Qinchuan cattle at National Beef Cattle Improvement Center Breeding Farm using 0.2% type II collagenase (1148090, Sigma), following the protocol described in our laboratorys previous studies [30,31,32]

Journal of Dermatological Science, 94(3), 135-142

Lenk S and Schonberger B: Penile deviation--our therapeutic concept

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