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HUBERMAN: But in addition to that, that you really think about using the minimal effective dose and that you use perhaps even the mildest of these different compounds in order to make sure that you don't desensitize any of the receptor pathways

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After, neurons were incubated in blocking solution containing anti-rabbit STAR635P (1:170 dilution, in-house conjugated rabbit Jackson ImmunoResearch 711-005-152 and STAR635P NHS ester Abberior 07679) and anti-mouse STAR580 (1:200 dilution, in-house conjugated mouse Jackson ImmunoResearch 715-005-151 and STAR580 NHS ester Abberior 38377) for 1 hour