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Physician-prescribed topical formulations deliver the peptide exactly where the benefits occur, at therapeutic concentrations (2 to 10 percent or more), without systemic distribution

doi: 10.1210/endocr/bqad133 144

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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )