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In phase I reactions, CYP1A1/2 and CYP3A4 oxidise estrogen to a 2-hydroxylated catechol estrogen, whereas CYP1B1 (which is constitutively expressed in the breasts, ovaries, adrenal glands, and uterus, as well as in several other tissues 13,14 converts it to 4-hydroxylated catechol estrogen 15,16

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Based on these results hGrx1-roGFP2 can be recommended as a reliable and specific biosensor for real-time spatiotemporal monitoring of the intracellular E GSH in P

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