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Cells were seeded on Falcon dishes at 37 C with 5% CO 2 in Dulbeccos modified Eagle medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin (Beyotime, China) and 100 mg/ml streptomycin (Beyotime, China) [26]

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Methadone , a full opioid agonist, has decades of evidence supporting its effectiveness, particularly in cases of high-severity opioid dependence