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Potassium phosphate buffer (100 mM, pH 7.0) was employed

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In brief, the gradient started with 5% buffer B and increased stepwise to 45% over 45 min, followed by a wash-out at 95% buffer B, all at a flowrate of 300 nl min 1

Back in 2010, the US Food and Drug Authority (FDA) concluded that increases in the incidence of carcinomas among rodents translated into a low risk for humans, because statistically significant increases occurred only at drug-exposure levels many times those anticipated in humans

This approach has been practically implemented via development of proteolysis-targeting chimeras (PROTACs) and molecular glue degraders, the latest discoveries in the field of biomedical research