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Furthermore, the association between PPI use and SIBO has conflicting results from multiple studies
Cell suspensions were lysed by freeze-thawing, and PV VLPs were purified from clarified supernatants by concentration through a 30% sucrose cushion followed by a 1545% sucrose gradient in 1 Dulbeccos phosphate-buffered saline (DPBS, Gibco), 20 mM EDTA, pH 7.0 (DPBS-EDTA) 15
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doi: 10.1093/ajcn/82.3.627
However, glutathione can slow down the process by fighting oxidative stress, which is regarded as one of the causes of early aging