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The GHK-Cu delays aging in Caenorhabditis elegans via coordinated regulation of mitochondrial function and activation of DAF-16/SKN-1 pathways

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To enhance the throughput and simplify the procedure, the assay has been adapted to a microplate format, replacing NBT with cytochrome c, and the absorbance is measured at 550 nm (MacDonald-Wicks et al

Besides deacetylation, there are also other types of PTMs identified in histone lysine (K) residues, which include crotonylation, malonylation and butyrylation [85, 86]