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Correct insertion yields a novel NcoI site generated by ligation of the blunted ends, and so NcoI (NEB) linearization of the supercoiled vector was used to determine correct insertion (Figure 1b, lower panel)

The more ICG that remains, the less glutathione in the liver, which directly correlates to liver damage

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For immunoblotting, samples were resolved on 8%, 10%, or 12% polyacrylamide minigels (Bio-Rad) and transferred onto a PVDF membrane (GE Healthcare Life Sciences, PA) by wet or semi-dry transfer

This historical context underscores its foundational connection to gut health and repair mechanisms