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The nodose ganglions (NGs) and brains were collected, postfixed in the same fixative for 2 h to overnight at 4 C, and incubated in phosphate buffer containing 30% sucrose for 48 h

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Subsequent studies established this activity as a tripeptide with an amino acid sequence glycyl-L-histidyl-L-lysine with a strong affinity for copper that readily formed the complex GHK-Cu

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doi:10.1016/j.phymed.2017.11.011