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Quantification of 2HG enantiomers 2HG quantification in SCD erythroblasts was performed as we previously described 24 by liquid chromatographymass spectrometry (LC-MS/MS) after derivatization using N-(p-Toluenesulfonyl)-L-phenylalanyl Chloride (TSPC) on the LC-ESI-MS/MS system

The Endogenous Decline Argument Plasma GHK falls from approximately 200 ng/mL at age 20 to approximately 80 ng/mL at age 60 in human cohorts 1 2

and HVA (+58%), p = 0.37