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Quantification of lipogenesis gene expression To quantify gene expression, we used the Pure Link RNA Mini kit (12183025, Invitrogen, USA), Hilden, Germany) to extract total RNA from untreated and treated HepG2 cells and used High-Capacity cDNA Reverse Transcription kit (4368813, Applied Biosystems, Foster City, CA, USA) and 2 g total RNA to prepare cDNA

Freeze-Thaw Cycling: This method helps to improve the encapsulation of sensitive biological molecules

[1] Some people with autism (~10-20%) seem to have L-carnitine metabolism disorders and for these patients, a diet high in carnitine and dietary supplementation with L-carnitine is beneficial

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PMID: 87 KlyneDMBarbeMFJamesGHodgesPW

Restoring these nutrients can help support brain development, immune function, and reduce oxidative stress