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Fluorescence was viewed using a charge-coupled device camera (DP72, Olympus, Melville, NY, USA) attached to a microscope system (BX51, Olympus) at 100 magnification

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Researchers became interested in this combination because early studies suggested that adding amylin pathway modulation to triple incretin agonism could produce synergistic effects beyond either component alone similar to how the CagriSema (cagrilintide + GLP1) combination outperformed individual components in clinical trials