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ROS assay was performed using ROS-Glo H 2 0 2 assay kit (Promega, MI) according to the manufacturers instructions
Vitamin C acts as an antioxidant which help to assist with collagen formation
The MT-CYB, CYC1, and UQCRFS1 encoded proteins constitute the electron transfer center of complex III
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Low ROS levels activate cell survival signaling pathways, such as the unfolded protein response and Nrf2 pathways, whereas high ROS levels activate cell death signaling pathways, such as apoptosis and necroptosis pathways 43