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In PCOS granulosa cells, despite the absence of evidence directly substantiating Cer-mediated pro-senescence effects, this bioactive sphingolipid has been demonstrated to elicit oxidative stress [12], mitochondrial dysfunction [22], and DNA damage [23], all of which are critical drivers of cellular senescence [24]

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After sub-culturing the enrichment eight times every 28 days, the culture was used as the initial inoculum for the Europa simulation experiments

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Functional consequences of sphingomyelinase-induced changes in erythrocyte membrane structure