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According to several reports, PI3K/AKT pathway activation upregulates genes that support vascular permeability, thrombogenicity, and inflammatory cascades by activating the NF-B pathway 54,74

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The entire sample was then passed through the stagetip twice before being eluted into a new LoBind Eppendorf using 80 L of 70% CH 3 CN/30% H 2 O/0.1% formic acid)