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Besides neurons, astrocytes and microglia express GLP-1Rs (see Vrang and Larsen, 2010 for a general overview) (Merchenthaler et al., 1999

If you are reconstituting with plain bacteriostatic water, the resulting solution should be clear

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The reagent was added to the assay plate according to the product instruction and allowed to incubate on an orbital shaker for 3 min at room temperature before subjecting the plate to the GloMax luminometer (Promega)