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Trends Mol Med 2015
Reverse transcription was performed with 2 g of total RNA with SuperScript II Reverse Transcriptase (Invitrogen) and random primers p(dN)6 (Roche Applied Science)
GIPR-Camper neurons of the AP were excited using a light-emitting diode (LED) light source (Dual Optoled, Cairn Research) at 435 nm for 50 ms every 3 s, and CFP and YFP emissions were simultaneously recorded after passing through an optosplit (Cairn Research
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Neither form has a clinically significant safety difference when paired with semaglutide
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