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FF (500 L) was mixed with methanol-acetonitrile (400 L, v/v = 2:1, with formic acid and succinic acid-2,2,3,3-d4), and KGN cells (110 7 ) were treated with methanol-water (v/v = 3:1, with 0.1% formic acid and succinic acid-2,2,3,3-d4), respectively

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Depression of mTOR signalling by siRNA interference inhibits phosphorylation of both p70S6K and 4E-BP1, which leads to apoptosis 13

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To evaluate ovarian response to exogenous gonadotropins, 7-week-old female mice were intraperitoneally injected with 5 IU of PMSG (AibeiBio, Nanjing, China)