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LaccID activity is specific to the mammalian cell surface In mammalian cells, LacAnc100 is active at the cell surface but fusions targeted to the cytosol, mitochondria or endoplasmic reticulum (ER) lumen are inactive (Supplementary Fig

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Pull-down assays with purified proteins and mutant SAP30BP Pull-down assays were performed using Strep-Tactin binding affinity of purified Twin-Strep-tagged CDK11 p110

E b , the value of BMI at baseline