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Ebselen has a wide range of specificity for various substrates, such as hydrogen peroxide, organic hydroperoxides (R-OOH)unstable by-products of lipid peroxidation, cholesterol hydroperoxides (CH-OOH)products of cholesterol peroxidation, and membrane phospholipids (Sies 1993)

Unlike classic dietary antioxidants (vitamin C, vitamin E) which localize to specific compartments, melatonin being both lipophilic and hydrophilic distributes through all cellular compartments, especially concentrating in mitochondria where the majority of ROS are produced

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At the same time, MOTS-c elevates cellular NAD levels and engages sirtuin pathways (e.g SIRT1), which are well-known longevity and metabolic regulators

These studies focus on biochemical signaling behavior rather than clinical application