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Estimation of the transcript level of the target genes Il1, Tnf- and Nf-B The total RNA was extracted from the lung tissue using the RNeasy mini kit (Qiagen)

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The plastic bottom of standard 24-well plates (Corning Costar, USA) was replaced by borosilicate glass coverslips (VWR International, USA)

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Open preliminary randomized prospective clinical trial of efficacy and safety of three different verapamil dilutions for intraplaque therapy of Peyronie's disease

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