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Finally, the samples were prepared for Western blotting analysis by lysing the brain homogenates on ice for 300 minutes in 100 mL of a lysis buffer composed of 120 mM NaCL, 40 mM Tris (pH 8), and 0.1% NP40, followed by centrifugation

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Anal Chem 93(1):228256 Hajam AH, Bashri G (2025) Strigolactones: key phytohormones in plantmicrobe interactions and development

doi: 10.1177/1533317520953041 235 CaiHYYangDQiaoJYangJTWangZJWuMNet al

GIP = glucose-dependent insulinotropic polypeptide