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Notably, EV-associated DAMPs have been found in stored red blood concentrates [238], suggesting that eryptosis-mediated generation of EVs might exert immunomodulatory DAMP-triggered effects

doi: 10.26719/2015.21.8.564

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Supernatant was discarded, and cells were subsequently incubated in 0.5 mL KRBH containing 0.1% BSA,1 mM glucose, and 10 M adrenaline (MCE, HY-B0447B) at 37 C for 1 h