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GO analysis of upregulated DEGs indicated that MASLD affected multiple aspects of muscle biology, primarily involving immune system activation, inflammatory response, and hormone- and cytokine-related processes (Fig

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Further work would be required to delineate whether histaminergic neuron activation in these conditions was mediated by the hypoglycemia or insulin itself

The GPx enzyme was then combined with these samples, and its activity was calibrated using the standard GPx-DTNB assay