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Samples (30 g protein) were resolved by 420% SDS-PAGE (Biofuraw Precast Gel, Tanon Science, Shanghai, China), transferred to polyvinylidene difluoride membranes, and incubated with one of the following antibodies: LC3 (88588s, Cell Signaling Technology, 1:1000), GPX4 (ab125066, Abcam, 1:1000), p16 (1003-1-AP, Proteintech, 1:1000)

Math check: Before drawing any dose, recalculate your concentration one more time

Plan light, balanced meals 12 hours before injection: lean protein, healthy fats, and vegetables in smaller portions than usual

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Prior to the surgical procedure, informed consent was obtained from each patient, and the use of samples for this research was approved by The Medical Ethics Committee of The First Affiliated Hospital of the University of Science and Technology of China

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