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After 96 hours, 10 l of the PrestoBlue reagent (A13261, Invitrogen) was added to each well, and plates were incubated at 37 C for 20 minutes

If you have sensitive digestion, start with a lower dose and increase gradually

Yip KS, Suvorov A, Connerney J, Lodato NJ, Waxman DJ (2013) Changes in mouse uterine transcriptome in estrus and proestrus

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Why isolate the terminal region rather than another part