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Nevertheless, for large probiotic-derived molecules, such as extracellular polysaccharides (ECPS) or extracellular vesicles (EVs), endocytosis is used as a receptor-mediated mechanism of active transport (67, 68)

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The resulting cell pellets were resuspended with 500 L of sorting buffer (DPBS -/- with 1 mM EDTA, 25 mM HEPES, and 0.2% BSA) and transferred to DNA Lo-bind tubes for FACS sorting

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The most accurate has been the vertebral heart score (VHS) method proposed by Dr