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These issues hinder reproducibility, validation, and regulatory approval
This reaction is catalyzed by glutathione peroxidase (GPx), producing oxidized GSSG, which is subsequently recycled back to GSH by glutathione reductase
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However, NAC is likely ineffective in increasing GSH levels in cells with dysfunctional GCL, in which -EC synthesis from Cys converted from NAC would be suppressed [5]
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