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Enzyme expression was induced by adding 100 M isopropyl -d-1-thiogalactopyranoside, and the cultures were incubated for another 20.5 h at 20 C, 300 rpm, 5 cm shaking diameter

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If hyperdiuresis is attempted, it must be monitored carefully and discontinued if patients don't respond favorably

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Reconstitute using an appropriate laboratory-grade solvent suitable for the intended research protocol