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The secreted proteins were purified from the harvested supernatants using a CaptureSelect Human Albumin Affinity Matrix (Thermo Fisher Scientific) packed in a 5-mL OPUS Chromatography Column (Repligen) 48

and Molecular Medicine 51, 110

An important limitation of this experiment is the lack of a conclusive determination of how LCn affects males' ability to reproduce

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Kim SE, Zhang L, Ma K, Riegman M, Chen F, Ingold I, et al

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