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J Pharmacol Exp Ther 340:134142 Alemayehu D, Zou KH (2012) Applications of ROC analysis in medical research: recent developments and future directions

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These observations are consistent with the relative affinities of the sensor variants determined in vitro ( Figure 1 ) and suggest robust and active maintenance of NADP redox state even under severe oxidative challenge, which is in stark contrast to previous observations of a highly volatile cytosolic NAD redox state as monitored by Peredox [13, 23]

Unbound material was washed out by 3x washes with binding buffer, and bound proteins were eluted with 2x SDS-PAGE sample buffer