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Our results indicated that neither GHK nor GHK-Cu showed cytotoxic activity at the range of concentrations tested while CuCl 2 and Cu(OAc) 2 showed substantial cytotoxicity to the HaCaT cells at the highest concentration assayed, i.e., at 5800 M, after 30 mins treatment

Next, they were immersed in phosphate-buffered saline containing 0.1% Triton X-100 for permeabilization and incubated with rabbit polyclonal anti-H2AX antibody (1:300

Sani M-A, Separovic F

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10.1074/jbc.M801925200 J