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These encompass the utilization of chemically modified siRNAs, such as cholesterolconjugated siRNAs, the employment of viral vectors (e.g., lentiviruses) to prolong the duration of action, and the combination with lipid nanoparticles (LNPs) to improve delivery efficiency [434, 435]
Recently, ten human patients with lumbar IVD disease treated with an intradiscal BMSC injection showed diminished pain and disability and a significantly elevated IVD water content (MRI) at the 1-year follow-up [98]
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We generally advise against pre-loading syringes for long-term storage
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