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After suspension of the pellet in a phosphate-buffered 218 mM sucrose (containing 4.9 mM potassium glutamate) the bacteria are pelleted through a 20% (w/v) iodixanol cushion (prepared by dilution of OptiPrep with the same buffered sucrose) at 14,000 g for 10 min

doi: 10.1016/j.freeradbiomed.2007.03.024, 83

(July 2006)

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This demonstrates how the incretin hormones are dependent on their receptor being present on islet beta-cells to mediate their insulinotropic effects

[1] [2] These mechanisms collectively improve glycaemic control and often result in weight loss as a secondary benefit