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26,600 g ) for 30 min at 4 C in a JA-25.50 rotor (Beckman Coulter), and cleared lysate was incubated for 45 min with 2.5 mL of Ni-NTA Superflow resin (Qiagen)

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Eur J Nutr 59(3):859872

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Up-regulation of nicotinamide phosphoribosyltransferase and increase of NAD + levels by glucose restriction extend replicative lifespan of human fibroblast Hs68 cells