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Briefly, an XhoI/EcoRI fragment containing a U6-shRNA cassette was amplified from pLKO.1 vector using high-fidelity PCR and ligated into XhoI/EcoRI-digested adenoviral shuttle vector pacAd5 K-NpA

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For patients starting fresh, or switching from semaglutide for efficacy reasons, tirzepatide remains the more proven option at comparable weight loss levels without the dysesthesia risk

10.1523/JNEUROSCI.2724-15.2015 J