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Research indicates that colonizing adult GF mice with a conventional microbiota significantly elevates their serum IGF-1 levels, correlating with heightened levels of the bone formation marker P1NP (N-terminal propeptide of type I collagen) and elevated bone formation rates, thereby facilitating both radial and longitudinal bone growth

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Materials and methods Cell lines and reagents The MCF-7 human breast cancer cell line was obtained from Dr Dean Edwards (University of Texas, San Antonio, TX, USA) and was maintained in phenol red RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 U/mL penicillin, 100 g/mL streptomycin, 1 non-essential amino acids and bovine insulin at 6 ng/mL

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Confirm HPLC purity above 98%

Injected testosterone shuts the testes down, because the brain sees enough hormone and stops sending its own signal