Upon fragmentation, each tag releases a unique reporter ion whose intensity reflects the peptides original abundance (Li et al., 2012)
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The blots were washed and then incubated with a secondary antibody conjugated to horseradish peroxidase (Jackson ImmunoResearch Laboratories, West Grove, PA, USA), and the blots were visualized using a western blotting luminol reagent kit (Santa Cruz Biotechnology, Santa Cruz, CA.)
On average, they can range from $20 to $50 per injection
A provider can tell you whether a peptide is appropriate for what you are trying to achieve, what the current evidence genuinely supports, what monitoring would be involved, and whether better-established options exist for your situation