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To quench the metabolism, 500 l of precooled -48 C 100% methanol (Sigma, Germany) was added to the cells, which were then incubated for 30 minutes at -80 C

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Oncotarget 6(28):25917 Wang Y, Patel A, Douglas RS (2019) Thyroid eye disease: how a novel therapy may change the treatment paradigm

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