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ROS levels in these cells were assessed using DCFH-DA staining followed by fluorescence microscopy, revealing that BV2-metformin-loaded PLGA reduced intracellular ROS compared to uncoated nanoparticles and restored mitochondrial membrane potential (MMP) via JC-1 staining

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Reactive oxygen and nitrogen species (ROS/RNS) can oxidize the aromatic rings of polyphenols, leading to their fragmentation and formation of degradation products such as di- and carboxylic acids